Table 1
Radioactivity associated with cells and cell components
Cells were cultured in media containing [14C]4F-GalNAc (0.5 μCi/mL, 100μM) for 38 hours. Radioactivity in culture supernatant and cells was measured. In addition, cells were lysed in 2% NP-40, and fraction of radioactivity in detergent-soluble and -insoluble fractions was determined; 10% TCA was applied to precipitate proteins in the NP-40–soluble fraction, and radioactivity associated with TCA precipitate was determined.
TCA indicates trichloroacetic acid.
