Role of CCR6 in mediating the antiviral activity of hBD2 in a spreading infection. CD4⁺ T lymphoblastoid cell lines JKT-FT7 (CCR6^low/no) and JKT-FT7 CCR6 GFP (CCR6⁺) were infected with X4 (IIIB) HIV. After infection, cells were treated with T20 (11.25 μg/mL; left) or with hBD2 (20 μg/mL; right). At the indicated time points, HIV p24 in tissue culture supernatant was quantified by ELISA, and the percentage of inhibition was calculated in reference to measured p24 in untreated control infections. Shown here are average percentages of inhibition values (± SEMs) of 4 independent experiments. *P < .05 (2-tailed t test) between homologous treatment groups for CCR6^low/no and CCR6⁺ cells.