Diagram of generating a lentiviral vector–transduced HPSC-transplanted hu-BLT mouse. CD34+ and CD34− cells isolated from a human fetal liver were transduced by either shRNA (EGFP+) or no shRNA (mCherry+) vectors. The transduced cells were solidified with Matrigel and implanted under a kidney capsule with a piece of human fetal thymus. Three weeks after the implantation, the mouse was irradiated and intravenously injected with vector-transduced autologous CD34+ cells. FL indicates human fetal liver segment; and FT, human fetal thymus segment.