Human hematopoietic differentiation of shRNA 1005-transduced HPSCs in multiple lymphoid organs. EGFP and mCherry reporter gene expression was examined in human CD45⁺ cells in gated lymphocyte population in multiple tissues. Samples were analyzed between 14 and 20 weeks after intravenous CD34⁺ cell injection. Mean percentage EGFP and percentage mCherry expression are shown in each organ. No significant difference was found (P > .05) between percentage EGFP⁺ and percentage mCherry⁺ cells in various tissues by Student t test. Data were generated from n = 4 to 10 individual animals from an aggregate of 8 donors. Error bar represents SD. PB indicates peripheral blood; Thy/Liv, transplanted human thy/liv organoid; BM, bone marrow; SL, spleen; LN, lymph nodes; SI LPL, small intestine lamina propria lymphocytes; and n, number of samples.