Analysis of cytokine profile by ^p190BCR-ABL–specific BM T cells. The frequencies of ^p190BCR-ABL–specific BM T cells producing IFN-γ, TNF-α, or IL-2, either as EM (dark bars) or CM (light bars), are shown as median percent positive cells, computed over all 10 patients. Results are expressed as percentages of CD8⁺ T cells (left) or CD4⁺ T cells (right). In addition, median magnitudes of specific cytokine-producing T cells for individual patients are reported on each column, either as EM (□) or CM (○). CD8⁺ or CD4⁺ T cells were gated on CD3⁺ events after passing through a small lymphocyte gate. Percentages of antigen-reactive EM or CM T cells were calculated as mean differences over unstimulated controls according to CSA methods.