Effects of protein S on neurologic and neuropathologic outcome in Tyro3, Axl, and Mer mutants after transient MCAO. Motor neurologic score, infarct volume, and edema volume (swelling) in Tyro3^+/+ and Tyro3^−/−, Axl^−/−, and Mer^−/− mice treated with vehicle or PS and subjected to 1-hour MCAO and 23-hour reperfusion (A-C), and in Tyro3^+/+ mice pretreated with W146 and sham-operated or subjected to 1-hour MCAO and 23-hour reperfusion and treated with vehicle or PS (D-F). The studied mice were the same as in Figure 4. Murine PS (0.2 mg/kg) or saline was administered via the femoral vein 10 minutes after the MCAO. (G) PS cerebrospinal fluid levels (nM) in wild-type mice 1 hour after intravenous administration of PS (0.2 mg/kg) or vehicle. Mean ± SEM; n = 6 mice per group. (H) A representative confocal scanning analysis of lectin-positive endothelium (red), Tyro3 immunodetection in the capillary endothelium (green), and aquaporin-4–positive astrocyte foot processes (blue) in brain in situ in a control mouse. Merged: yellow. Bar represents 4 μm. Chart: Tyro3 relative signal intensity (green) plotted over the endothelial-specific lectin signal intensity (red). A indicates abluminal side; L, luminal side.