MHC-I, MHC-II and LAMP-2 distribution after AC uptake in IL-4 DCs. CLSM analysis (central optical sections) of IL-4 DCs incubated or not with anti–LOX-1 mAb before 24-hour coculture with PHK26-PE–labeled ACs. DCs were fixed, made permeable, and then stained with FITC-conjugated anti–MHC-I (A), anti–MHC-II (B), or with LAMP-2 antibodies (C). Colocalization of ACs with the mentioned molecules is shown in yellow; scale bars, 10 μm. Data are representative of 3 independent experiments.