Cellular distribution of DCIR. (A) Flow cytometry analysis of DCIR expression on peripheral blood mononuclear cells. Circulating mononuclear cells were stained with 10 μg/mL anti-DCIR mAb followed by phycoerythrin-conjugated goat anti–mouse IgG. Cells were incubated with anti-CD19, anti-CD4, anti-CD8 (for lymphocytes), anti-CD16, anti-CD56 (for NK cells) not shown, anti-CD14 mAb (for monocytes), or with anti-CD11c, anti–HLA-DR, and anti-CD123 mAb (for pDCs or mDCs) and analyzed by flow cytometry. Data are representative of 3 independent experiments performed on 3 different donors. (B) Expression analysis of DCIR by flow cytometry on skin-derived DC subsets: epidermal LCs, dermal CD1a⁺ DCs, and dermal CD14⁺ DCs. (C) Human epidermal sheets, stained with anti-DCIR and analyzed by fluorescence microscopy, revealed the expression of DCIR on HLA-DR⁺ LCs. Image was captured using an Olympus BX51 microscope with Planapo 40×/0.95 dry objective, Photometrics Coolsnap HQ camera, and Metamorph software Version 6.2r6. Channel separation was done in Adobe Photoshop CS. (D) Expression analysis of DCIR by flow cytometry on CD34⁺-derived DC subsets CD1a⁺ LCs and CD14⁺ DCs.