DCIR allows cross-presentation of proteins by LCs. (A) Skin-derived LCs from an HLA-A201⁺ donor were targeted with 8nM each of anti-DC.doc-coh.FluMP, IgG4.doc-coh.FluMP conjugate mAbs, or free FluMP matured with CD40L, and cocultured with autologous CD8⁺ T cells. Ten days later, CD8⁺ T-cell expansion was evaluated by specific HLA-A201–FluMP(58-66) tetramer staining. Data are representative of 2 independent experiments performed with cells from 2 different donors. (B) IFN-γ levels as measured by Luminex in the culture supernatant of CD8⁺ T cells expanded for 10 days by autologous skin LCs targeted with anti–DCIR.doc-coh.FluMP or IgG4.doc-coh.FluMP conjugate mAbs. Graph represents mean ± SD; n = 3.