DCIR is a global target for all blood DC subsets. (A) Blood-derived mDCs from an HLA-A201⁺ donor are targeted with 8nM, 0.8nM, or 80pM each anti–DCIR.doc-coh.FluMP (clone 24A5), IgG4.doc-coh.FluMP conjugate mAbs, or free coh.FluMP, matured with CD40L and cocultured with autologous CD8⁺ T cells. Ten days later, CD8⁺ T-cell expansion was evaluated by specific HLA-A201–FluMP(58-66) tetramer staining. Data are representative of 3 independent experiments. (B) Blood-derived pDCs from an HLA-A201⁺ donor were targeted with 8nM, 0.8nM, or 80pM each anti–DCIR.doc-coh.FluMP (clone 24A5), IgG4.doc-coh.FluMP, or free coh.FluMP, matured with CD40L and cocultured with autologous CD8⁺ T cells. Ten days later, T-cell expansion was evaluated by specific HLA-A201–FluMP(58-66) tetramer staining. Data are representative of 3 independent experiments. (C) Percentage of FluMP-specific CD8⁺ T cells induced by 8nM DCIR.doc-coh.FluMP complex mAb-targeted mDCs or pDCs. Graph represents results of 3 independent experiments using 2 different clones of DCIR mAb. P = .02.