Functional characterization of the Galc−/− niche. (A,D) Experimental schemes. (B,E) Survival curves of Galc−/− mice (FVB/Twi in panel B and Twi in panel E) after the intravenous or intraperitoneal transplantation of GFP⁺Galc+/+ HSPC or KLS or total BM (TBM) or HSPC plus GFP⁻ Sca1⁻ BM cells, as indicated. KLS intravenous (WT): KLS transplanted intravenously in wild-type recipients. n ≥ 3 in each group. (C,F) Engraftment failure frequency and donor chimerism measured in the same experimental groups as in panels B and E. Average values ± SD are reported. *P < .05, **P < .01 on 1-way ANOVA.