The minor H antigen recognized by J 47 is expressed on AML LICs. Cohorts of sublethally irradiated NOD/SCID mice were inoculated with 5 × 106 AML cells alone or with AML cells that had been cocultured overnight with clone J 7 (specific for a minor H antigen not expressed on the AML) or with clone J 47 at an E/T ratio of 10:1. (A) FACS analysis of BM obtained at 6 weeks and stained for human CD45+CD33+ cells in individual mice that received AML alone (top panel); AML and the control clone (middle panel); and AML and J 47 (bottom panel). Data are shown for 3 representative mice (of 5-10 in each cohort). The mouse on the righthand side of the middle panel died just before the 6-week evaluation, and the BM was obtained postmortem, accounting for higher nonspecific staining in the lower right of the flow plot. (B) Leukemic engraftment in the BM at 3 (■), 6 (□), and 8.5 (▧) weeks after transplantation evaluations in mice in each cohort. Bars represent SD. (C) Leukemic engraftment in the spleen killed at 8.5 weeks after transplantation in mice in each cohort. (A-C) The data are representative of 2 experiments with 2 different AML samples that express the minor H antigen recognized by J 47.
