PKB induces expression of integrins. CD34⁺ cells were cultured in presence of the PKB inhibitor VIII for 4, 24, or 48 hours. Flow cytometric analysis was performed to determine the level of (A) CD18 (n = 5) and CD49d (n = 5). (B) Data were depicted as fold induction compared with untreated cells. (C) CD34⁺ cells were cultured in the presence of the PKB inhibitor VIII for 24 or 48 hours. Flow cytometric analysis was performed to determine the expression level of CD18 (n = 3) and CD49d (n = 3) directly after isolation and after a 48-hour culture period. (D) Data were depicted as fold induction compared with cells analyzed immediately after isolation. (E) CD34⁺ cells were cultured in the presence of the PKB inhibitor VIII for 2, 24, or 48 hours. Flow cytometric analysis was performed to determine the intracellular level of CD18 (n = 5) and (CD49d (n = 5). (F) Data were depicted as fold induction compared with untreated cells. Error bars represent SEM. Samples significantly different (P < .05) are indicated with horizontal lines and asterisks.