Inhibition of PKB induces homing of human hematopoietic progenitors in β2-microglobulin^−/− NOD/SCID mice. CD34⁺ cells, cultured in the presence of SCF and FLT-3L, were transduced with empty vector alone or myrPKB. Three days after transduction, cells were injected into β2-microglobulin^−/− NOD/SCID mice. Four, 22, and 44 hours after injection, mice were killed, and the percentages of eGFP⁺ cells migrated to the (A) bone marrow and (C) spleen were determined by flow cytometric analysis and flow count beads (n = 3). (B,D) Data obtained 22 hours after injection was also depicted as fold induction compared with controls (n = 5) (E,I) CD34⁺ cells were cultured in the absence or presence of the PKB inhibitor VIII for 24 and 48 hours, on which cells were stained with cell tracker and injected into β2-microglobulin^−/− NOD/SCID mice. Twenty-two hours after injection, mice were killed, and the percentage of cell tracker–positive cells in the (E) bone marrow and spleen was determined by flow cytometric analysis and flow count beads (n = 5). (F,H) The percentage of injected cells migrated to the bone marrow and spleen was calculated. (G) Data were depicted as fold induction compared with cells cultured in absence of the PKB inhibitor. Error bars represent SEM. Samples significantly different are indicated with horizontal lines and asterisks.