In vitro and in vivo phagocytosis of YG beads by murine peritoneal macrophages. In vitro (A,B top panel) and in vivo (C-D bottom panels) phagocytosis of YG beads by stimulated peritoneal macrophages from wild-type and P2X7-knockout C57BL/6 mice. For in vitro assay, cells were treated with or without 1mM ATP for 15 minutes before the addition of beads. For in vivo assay, mice were injected intraperitoneally with either 1 mL of PBS or 5mM ATP followed 15 minutes later by injection of 1-μm YG beads. The mice were killed after 30 minutes, and peritoneal exudates were collected. Monocyte-macrophages were gated by forward scatter-height and side scatter-height (A-B) plus CD11b+ (C-D). Doublets were excluded by side scatter-height and side scatter-area gating (supplemental Figure 9). *P < .05; **P < .02 (n = 5).
