HLA-B35 EBNA1-specific T cells are dominant over EBNA1-specific T cells with other HLA class I alleles. Polyclonal EBNA1-specific IFN-γ CD8⁺ T-cell responses were analyzed for HLA class I associations. HLA class I alleles with a frequency in our control and patient samples of greater than 3 were analyzed, and EBNA1-specific IFN-γ⁺ CD8⁺ T-cell responses were compared using Mann-Whitney test. Although EBNA1-specific IFN-γ⁺ CD8⁺ T cells were expanded from all of the HLA class I alleles present in our samples, HLA-B35 EBNA1-specific T cells were statistically dominant. Error bars represent SEM.