MP-IVM investigations on T-cell interstitial motilities. Motility parameters were measured 18 to 24 hours after adoptive transfer of WT and α_L-I306A T cells in C57BL/6 mice. (A) Mean 3D instantaneous velocity (3D-IV). (B) Meandering index. (C) Motility coefficient. (D) Tuning angles. (E) FACS histograms showing a staining of PLN T cells with FITC-labeled anti–LFA-1 mAb M17/4. T cells were isolated from either mice that had been administered with unlabeled M17/4 (middle panel) or those that had been mock treated (top panel). Please note that prior in vivo administration of unlabeled M17/4 competed for LFA-1 with FITC-M17/4. (F-G) Motility parameters after LFA-1 inhibition with M17/4. M17/4 treatment affected neither meandering index (F), mean 3DIV (G), nor motility coefficient (H) of WT and KI cells. (A-H) A representative result from 3 independent experiments is shown. (C,H) Data are expressed as the mean values ± SEM. Data are expressed as mean values ± SEM (B,F-G). The line in the box-plot indicates the mean values, and the top and the bottom of the box represent the maximum and minimum values, respectively. The data are from 3 independent experiments. Two-tailed Student t test was used for statistical analyses. Statistical significance was defined as *P < .05 or ***P < .001.