Memory phenotype analysis of MAGE-A2_212-220–specific CD8⁺ T-cell response detected in patient no. UPIN18 after treatment with AZA/VPA. The figure shows flow cytometry plots after magnetic enrichment in which the enriched cells were then costained with antibodies against CCR7, CD45RA, CD27, CD28, and CD57. This enabled a full characterization of T-cell differentiation. The gating strategy used is displayed in fluorescence-activated cell sorter plots (i). Fluorescence-activated cell sorter plot (ii) shows the number of MAGE-A2_212-220–specific CD8⁺ T cells (region P5) that expressed CD137. These cells were then plotted against CCR7 and CD45RA (iii). Cells that were CD45RA⁻CCR7⁻ (region P6) were further plotted against CD27 and CD28 (iv) and CD27 and CD57 (v). Cells that were CD45RA⁺CCR7⁻ (region P7) were further plotted against CD27 and CD28 (vi) and CD27 and CD57 (vii).