Analysis of MAGE-A2212-220–specific CD8+ T-cell response detected in patient no. UPIN18 with the IFN-γ cytokine secretion and enrichment assay. Fluorescence-activated cell sorter plot (i) shows the number of CD8+ IFN-γ–secreting T cells detected in the unstimulated sample after magnetic enrichment compared with the sample stimulated with the MAGE-A2212-220 peptide (ii).