The effect of anti-VEGF on the recruitment of KDR-expressing lymphocytes in vivo. SCID mice with human skin transplants were humanized by adoptive transfer of human PBMCs intraperitoneally. At the time of humanization, and every other day, the mice received human IgG or humanized anti–human VEGF (5mg/kg in 100μl saline). After 14 days, the skin grafts were harvested and were analyzed by H&E staining and by immunofluorescence. Illustrated in the lefthand panels are representative photomicrografts of skin grafts harvested from mice treated with control IgG. The photomicrografts illustrated in the right panels are representative of skins harvested from mice treated with anti-VEGF. Expression of CD3 is illustrated in green, KDR in red, and the merged images (bottom panels, yellow color) illustrate coexpression of KDR with CD3 on T-cell infiltrates. Magnification of all micrographs, ×400. Boxed insets, magnification × 800. Representative of n = 4 mice in each group.