Mechanism of action of AT9283 in BaF3 BCR-ABL cells and human CML cell lines. BaF3/wt-BCR-ABL, BaF3/T315I, BV173, and K562 were incubated with the indicated concentration of AT9283, or vehicle control, for 24 hours before preparation for immunoblotting with the indicated antibodies. The blots shown are representative of at least 2 independent experiments in each cell line.