AMPKα subunit expression in human platelets and effect of thrombin. (A) Expression of AMPKα1 and AMPKα2 in washed human platelets and the effect of thrombin (0.03 or 0.3 U/mL, 10 minutes) on AMPK phosphorylation of Thr172. (B) Effect of thrombin on the phosphorylation of ACC on Ser79. The bar graphs summarize data from 4 independent experiments; *P < .05 and **P < .01 vs solvent (Sol)–stimulated platelets.