The rhTSLP promotes DC maturation. Decidual DCs and peripheral blood moDCs were stimulated with TSLP or LPS, or cultured in medium only. CD40, CD83, OX-40L, CD80, CD86, and HLA-DR expression was determined by flow cytometry. Filled histograms represent staining of the cells with the markers on the top of histograms; and open histograms, the isotype control (A). The numbers in the histograms indicate the percentage of positive-staining cells in DCs (B).TSLP up-regulates CD40, CD83, OX-40L, CD80, CD86, and HLA-DR expression on DCs compared with the control. The phenotypic changes on moDCs were similar to dDCs. Numbers indicate the percentage of positive-staining cells on DCs (B). The results shown are from 4 independent experiments with 4 cases. Data are mean ± SD. The picture is a representative one.