TSLP-treated DCs induce CD4⁺ T cells to polarize toward a T_H2 bias. The 2 kinds of DCs were exposed, respectively, to 15 ng/mL rhTSLP (TSLP-DCs), 50 ng/mL rhIL-7 (IL-7-DCs), or 10 μg/mL LPS (LPS-DCs) for 24 hours, washed 3 times, and then cocultured, respectively, with decidual CD4⁺ T (dCD4⁺ T) or peripheral CD4⁺ T (pCD4⁺ T) cells for 5 days. Thereafter, the T cells were transferred to a new 96-well round-bottom plate precoated with anti-CD3 and anti-CD28 and cultured for 24 hours. The cytokine secretion of the CD4⁺ T cells was determined by ELISA. IL-4, IL-5, and IL-10 secretions of the decidual CD4⁺ T cells cocultured with the TSLP-treated decidual DCs are highly up-regulated, but no such trend is observed for IFN-γ and TNF-α production, compared with the primary DCs. The moDCs pretreated by rhTSLP induce peripheral CD4⁺ T cells (pCD4⁺ T) to produce higher levels of IL-4, IL-5, and TNF-α, but lower levels of IL-10 and IFN-γ. Data are the means ± SD from 4 independently conducted experiments with 4 samples.