PLC-γ2 phosphorylation in wild-type and TULA-dKO murine platelets. (A) Isolated wild-type and TULA-dKO platelets were stimulated with 200 ng/mL convulxin for 0, 30, 60, 120, and 240 seconds, the protein precipitated, analyzed by Western blotting, and pY759 PLC-γ2 and total PLC-γ2 probed. (B) Same as panel A, except that platelets were stimulated with 50 μg/mL collagen. (C) Isolated wild-type and TULA-dKO platelets were stimulated for 2 minutes with 50, 100, 200, 400, or 800 ng/mL convulxin, processed for Western blotting, and probed for pY759 PLC-γ2 and total PLC-γ2 probed. (D) Same as panel C, except that platelets were stimulated with 25, 50, or 100 μg/mL collagen. Ratios were derived by dividing pY759 PLC-γ2 band quantitation by total PLC-γ2 band quantitation. Convulxin blots are representative of 3 experiments.