Ca²⁺ mobilization in wild-type and TULA-dKO platelets. FURA-2-AM-loaded murine platelets were stimulated with various doses of CRP in the presence of 100μM 2MeSAMP, 10μM MRS2719, 150nM echistatin, 10μM indomethacin, and 2mM probenecid, and the Ca²⁺ mobilization was measured using a spectrofluorometer. The change in intracellular calcium was then measured, plotted against dose of CRP, and fitted with a hyperbolic curve. Data points are constructed from 3 independent experiments.