Platelet aggregation and dense granule secretion in wild-type and TULA-dKO platelets. (A) Isolated wild-type and TULA-dKO platelets were stimulated with 100 ng/mL or 200 ng/mL convulxin, and the change in light transmittance was observed. (B) Same as panel A, except that platelets were stimulated with 1, 2, or 4 μg/mL CRP. (C) Same as panel A, except that platelets were stimulated with 50, 100, or 300μM AYPGKF. (D) Simultaneous to platelet aggregation studies, dense granule secretion was measured by monitoring adenosine triphosphate release using a luciferin-luciferase reagent; 50, 100, and 200 ng/mL convulxin were used. (E) Same as panel D, except that platelets were stimulated with 1, 2, or 4 μg/mL CRP. (F) Same as panel D, except that platelets were stimulated with 50, 100, or 300μM AYPGKF. All traces are representative of 3 experiments.