AKO mast cells demonstrate mitochondrial damage. (A) Mitochondrial membrane potential was assessed by JC1 staining. Data are representative of 3 independent experiments. (B) Ratios of JC-1 red versus green fluorescence. (C) Mitochondrial mass was detected by MitoTracker green staining. Data are representative of 3 independent experiments. (D) Numerical data of panel C. Data are representative of 3 independent experiments. (E) Transmission electron microscopy images of WT and AKO mast cells. (F) AKO mast cells showed elevated levels of intracellular ROS. Mast cells were cultured in the cytokine-free medium for 4 hours and then labeled with CM-H2DCFDA, and the levels of ROS were detected by flow cytometry. Data are representative of 3 independent experiments. (G) A heatmap of gene-chip array and gene cluster analyses of WT and AKO mast cells. (H) Analysis of apoptosis in WT and AKO mast cells. Mast cells were cultured in the WEHI-3–conditioned medium for 4 weeks. BMMCs were treated with 100 μM H₂O₂ overnight or using medium (M) as a control, and cellular apoptosis was detected by the use of annexin V and 7-AAD staining. Data are representative of 3 independent experiments.