Increased miR-181a activity in primary LECs results in reversion of LEC phenotype to BEC. Expression of Vegfr3 (A), Itga9 (B), Cd34 (C), Nrp1 (D), Vcam1 (E), and Pecam1 (F) mRNA relative to Actb, as assessed by real-time RT-PCR in primary embryonic LECs transfected with a negative control miR-neg (gray bars) or miR-181a (black bars); n = 3. Vegfr3: *P < .015. Itga9: *P < .017. Cd34: *P < .025. Nrp1: *P < .001. Data are mean ± SEM and are representative of 3 independent experiments. P values were calculated using Student paired t test.