IF and flow cytometric analyses of AML patient samples using the T26 mAb. (A) Representative IF images of bone marrow cells from type A (top panel), type B (middle panel), NPMc⁺ AML or wt NPM1 AML (bottom panel) patients, as indicated. Patient identification numbers are indicated as in Table 1. (B) Representative IF images of NPMc⁺ blasts with nuclear + cytoplasmic (top panels) or nucleolar + cytoplasmic (middle panels), and cytoplasmic T26 staining in NPMc⁺ leukemic cells with segmented nuclei (bottom panels). T26 staining, DAPI staining, and merged channels (Merge) are shown. (C) Representative example of flow cytometric analysis of a bone marrow sample from an NPMc⁺ AML patient (Pt # 10). Dot-plot showing the blast gate set on forward scatter/side scatter (left) and the histogram displaying the fluorescence of cells within the blast gate (right). Patients' lymphocytes present in the sample were used as a negative control.