CD1d and HIV-1 Vpu colocalize in early endosomal compartments. (A-B) Costaining of CD1d and EEA-1 (A) or LAMP-1 (B), respectively, in permeabilized 293T cells transiently coexpressing CD1d and Vpu-EGFP. Filled arrowheads (A) indicate vesicles staining positive for CD1d, Vpu, and EEA-1. (B) Filled arrowheads indicate colocalization of CD1d and LAMP-1; open arrowheads indicate colocalization of CD1d and Vpu. Squares indicate the section shown in higher magnification. Original magnification of all images, ×630; all data are representative of at least 3 independent experiments. Scale bars, 15 μm.