The immune reconstitution of CD3⁺ Vδ2pos and CD3⁺ Vδ2neg γδ T cells in patients during the first 24 months after SCT. (A) Absolute numbers as cells/μL of Vδ2pos and Vδ2neg γδ T cells. (B) Absolute numbers of naive Vδ2pos and Vδ2neg γδ T cells were determined during 3 to 24 months after SCT. Isolated PBMCs were stained with anti-CD3, anti–TCR-γδ, and anti-Vδ2 antibodies and analyzed by flow cytometry. Samples were gated on live lymphocytes as side scatter versus forward scatter followed by gating on CD3⁺ T cells. Naive phenotype was defined as CD45RA⁺, CD27^bright, and CD11a^dull. Dashed lines represent the upper and lower normal range generated from 45 healthy donors; the dotted line is the median value of the normal range. The horizontal bars represent the median value from patient samples for each time point.