Increased neutrophil apoptosis in patients with G6PC3 deficiency resulting from homozygous inheritance of G6PC3 G260R. Neutrophils from both patients and 2 healthy donors (HD) were cultured at 37°C for 2 hours (left panels) or 4 hours (right panels) in medium alone or medium with the addition of tumor necrosis factor (TNFα) or thapsigargin. Flow cytometry was then performed. Apoptotic cells are defined as annexin V⁺. Dead cells are defined as propidium iodide–positive (PI)⁺. Data are from a single experiment performed after the patients were treated for 7 months with G-CSF.