Figure 4
![Figure 4. Protein 4.1 and minispectrin-dependent ternary complex formation. (A) Minispectrin-dependent binding of [125I]-protein 4.1 to F-actin. [125I]-Protein 4.1 (0.38μM), F-actin (2.38μM as G-actin), and increasing amounts of either minispectrin or minispectrin-Δ13 were combined, and the bound 4.1R was measured after pelleting the F-actin. (B) Protein 4.1-dependent binding of [125I]-minispectrin or [125I]-minispectrin-Δ13 to F-actin. Increasing amounts of protein 4.1R were added to the [125I]-labeled minispectrins (0.54μM) and F-actin (2.38μM), and each bound minispectrin was measured after pelleting the F-actin. Binding of the mutant minispectrin is impaired under both conditions.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/14/10.1182_blood-2009-12-260612/4/zh89991058100004.jpeg?Expires=1735570909&Signature=rZvuIvR01Z53Bkpl89UIWYFi7LpV5hIT4VcpJU8d~iw3hnHwJav3oAPYAWp7Ik3Z-Sux2sQT1ZF53HON5HvCS1Yz1AzRzG3NDsWphBFXrcCDqSclz1VB6Hwj4LhyBfgWhWwZRzKW5L7~B1779l4nzoHdIwYmn03hh0KncXr3QpcWo2p53ntYJwQ8nq9N1KRJNIUgLKGRRCXCHvdSKIHpqlsGLJdr~P7C9HUTAgW~U5DsDDztSYDu6J4P-TAGeMJMC4u1z-UvYbRTdw5UUFGbberSyhpPaOUHU-WnQk33GoCpgxqO1oa7k-wLX1CMAoCwOL0Hqr7PmQ913KQrhm7x5A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Protein 4.1 and minispectrin-dependent ternary complex formation. (A) Minispectrin-dependent binding of [125I]-protein 4.1 to F-actin. [125I]-Protein 4.1 (0.38μM), F-actin (2.38μM as G-actin), and increasing amounts of either minispectrin or minispectrin-Δ13 were combined, and the bound 4.1R was measured after pelleting the F-actin. (B) Protein 4.1-dependent binding of [125I]-minispectrin or [125I]-minispectrin-Δ13 to F-actin. Increasing amounts of protein 4.1R were added to the [125I]-labeled minispectrins (0.54μM) and F-actin (2.38μM), and each bound minispectrin was measured after pelleting the F-actin. Binding of the mutant minispectrin is impaired under both conditions.
