Figure 6
![Figure 6. Comparison of the binding of [125I]-spectrin α18-21EF, [125I]-βABD1-4, [125I]-minispectrin, and [125I]-minispectrin-Δ13 to F-actin in the presence of protein 4.1R. The spectrin fragments and protein 4.1 were all 0.38μM. F-actin was 2.38μM in G-actin monomers. Error bars represent the range of 2 to 5 independent experiments for each protein. Neither the isolated α-spectrin EF domain nor the β-spectrin actin-binding domain binds to actin at these concentrations, whereas the assembled minispectrin binds robustly.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/14/10.1182_blood-2009-12-260612/4/zh89991058100006.jpeg?Expires=1735570177&Signature=VTRexvSkOlgrk4L~kB~T56ZZRsJ5I18F-nnmNq4fczBAAisdXo2hxgbT5N27sqQflSXMOd0mhijTAYOjS5bMsN1FQvBaXKycUg2crX~MkX5sbGZjAaIGETCkj56Nn7diyM7487XeD24vRNipnrNS29ftncREDUaW603gx5t0L6Hp2ZusG3wrAJUK8mNVSGf3quNgIbNc1nSDK8PftONoXyp1jVfPsehnQSkd5-n9MVLZYX1g~PeRz6~oec76mkJiC-xvnfOk2KIIyen48hkyhN23Fl16gMMEOBAVSOaav0UJPIR3utUg9fF5NimVg5of6dobt~NFZkjOzZYf2ErxoQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Comparison of the binding of [125I]-spectrin α18-21EF, [125I]-βABD1-4, [125I]-minispectrin, and [125I]-minispectrin-Δ13 to F-actin in the presence of protein 4.1R. The spectrin fragments and protein 4.1 were all 0.38μM. F-actin was 2.38μM in G-actin monomers. Error bars represent the range of 2 to 5 independent experiments for each protein. Neither the isolated α-spectrin EF domain nor the β-spectrin actin-binding domain binds to actin at these concentrations, whereas the assembled minispectrin binds robustly.
