Hematopoietic cell engraftment and multilineage development in the spleen. The level of human CD45⁺ cells (A), CD19⁺ B cells (B), CD19⁺IgM⁺ B cells (C), CD3⁺ T cells (D), and CD56⁺ NK cells (E). Lineage engraftment (B-E) expressed as frequency of human CD45 cells. *P < .05, **P < .01, ***P < .001 by Wilcoxon rank-sum test. ● represents experiment 1 females; ○, experiment 1 males; ■, experiment 2 females; and □, experiment 2 males. N = 5 to 7 per group.