Tissue-specific recovery of iNKT cells in IL-15Rα BM chimeras. (A-B) Tissue-resident iNKT cells or iNKT subsets were identified in the thymus, spleen, and liver of IL-15Rα^−/− BM chimeras 8 to 10 weeks after irradiation and BM reconstitution by flow cytometric analysis. (A) Representative flow cytometric plots of CD1d tetramer⁺CD3⁺ cells from the indicated tissues. (B) Absolute numbers of CD1d tetramer⁺ cells in the respective tissues from the indicated groups. Error bars represent SEM, and the numbers above bars represent the percentage of WT levels. *P ≤ .05. Data are the average from 3 independent experiments: n = 9 mice per group for the thymus and n = 8 for the liver and spleen. Statistic significance was examined by unpaired Student t test.