Megakaryocyte apoptosis and Fas ligand expression is unaffected in Dscr1 transgenic mice. (A) Flow cytometry of TUNEL⁺ CD41⁺ bone marrow–derived megakaryocytes from wild-type (WT) and Dscr1 Tg mice. Quantification of CD41⁺TUNEL⁺ cells (right). (B) Quantitative polymerase chain reaction analysis of Fas ligand mRNA in sorted CD41⁺ bone marrow cells (n = 3). (C) Western blot analysis of Fas ligand expression in sorted CD41⁺ bone marrow cells demonstrate no difference in WT vs Dscr1 Tg megakaryocytes. β-actin was probed as a loading control. (D) Quantitative polymerase chain reaction analysis of Fas ligand mRNA in sorted populations of small and large CD41⁺ bone marrow cells (n = 3).