Calcineurin-NFAT activation is suppressed in human trisomy 21 fetal liver megakaryocytes. (A) Megakaryocyte growth in liquid culture shown as fold change of CD41+ cells (n = 5 euploid (wild-type [WT]) and 5 trisomy 21 [T21]). Results are shown as mean ± SEM. (B) Quantitative polymerase chain reaction analysis of DSCR1 mRNA in euploid (WT) and T21 fetal liver megakaryocytes relative to cyclophilin; P = .035 (n = 5 WT and 5 T21). (C) Western blot indicates increased expression of DSCR1 and phosphorylated NFATc2 (p-NFATc2) in T21 fetal liver megakaryocytes compared with control. (D) Control and T21 fetal liver megakaryocytes were immunostained for NFATc2 (red) and Hoechst (blue) before and after ionophore treatment. Arrows denote megakaryocytes.