787F mutation does not affect c-Kit expression, SCF binding, and c-Kit shedding. (A) BMMCs (0.5 × 10⁶) from wild-type and Kit^787F mice were stained with PE-labeled c-Kit–specific antibodies, and MFI was measured by FACS (n = 7-9; *P = .038). Means are indicated as lines. (B) To compare SCF binding, cells were preincubated on ice for 2 hours, washed, and recombinant mouse SCF was added. One representative experiment from 3 is shown. BMMCs from 3 independent wild-type and Kit^787F BMMC cultures were analyzed. (C) c-Kit shedding was comparable in wild-type and Kit^787F BMMCs and was induced by incubation with PMA (100 ng/mL) for 30 minutes at 37°C. Cells were stained with APC-labeled c-Kit–specific antibodies and analyzed by FACS. Gray histograms show c-Kit expression in untreated controls, white histograms show c-Kit expression after PMA-induced shedding, dashed-line histograms show isotype control. One representative experiment from 3 is shown.