Reduced eosinophil development during CD70-mediated immune activation is IFN-γ dependent. (A) Representative plots of Siglec-F staining on bone marrow and spleens of WT, CD70TG, IFN-γ^−/−, and CD70TGx IFN-γ^−/− mice showing eosinophils as SSC^hiSiglec-F⁺ cells (percentages are shown). (B) Absolute numbers of eosinophils in the bone marrow (2 femurs and 2 tibiae) and spleens of these mice. (C) Absolute numbers of total T cells and T-cell subsets (percentages are shown) in the bone marrow of WT, CD70TG, IFN-γ^−/−, and CD70TGx IFN-γ^−/− mice. EM indicates effector memory; and CM, central memory. (B-C) Data are the mean of 3 individual mice per group. Data are mean ± SD. Experiments were performed at least 3 times with similar results. *P < .05; **P < .01; ***P < .001.