Diagrams of the constructs used to generate transgenic mice. All constructs are flanked by the chicken β-globin HS4 insulator (cHS4). The CMV enhancer/chicken β-actin promoter (CMV-β-ac) is used to express either the wild-type RPS19 cDNA (RPS19) or an RPS19 cDNA with the R62W point mutation (RPS19R62W). After the RPS19 stop codon, the 3′ end of the human γ-globin gene, including 52 bp of exon 2 (out of frame), IVS2, exon 3, and the 3′untranslated region (γ-globin IVS2 3′LTR) are attached to provide sequences for splicing, poly A addition, and mRNA stability. (A) Constructs that allow constitutive expression of the RPS19 transgenes. (B) Constructs that allow conditional expression of the RPS19 transgenes after Cre-mediated excision of the PGK-Neo sequences (Neo^R) flanked by Lox P sites that were inserted between the CVM enhancer/chicken β-actin promoter (CMV-β-ac) and RPS19 cDNAs.