Iron overload induces inflammatory changes. (A) Western blot for protein carbonyl content from bone marrow of placebo and iron dextran-treated mice. An overlapping series of bands were present in the iron-overloaded animals, indicating increased carbonyl content in a number of bone marrow proteins consistent with increased ROS in the bone marrow of these mice. (B) Serum TNF-α and IL-6 concentrations in mice treated for 2 months with high-dose (▬) or low-dose iron dextran () and placebo (▭). Serum cytokines increase in a dose-dependent fashion. Data are mean ± SD. *P < .01.