RA has varying effects on other genes expressed in the posterior mesoderm. (A). Flat-mounted 12-ss embryos are shown after whole-mount in situ hybridization with the probes indicated. Treatment with 10−6M RA was applied to wild-type AB embryos from 90% epiboly until 12 ss. Representative embryos are shown from a single experiment. Replicate experiments showed comparable results in at least 2 other experiments with each probe. All the embryos from a given treatment group had similar expression patterns. (B). Whole-mounted embryos at 24 hpf. Treatment with 10−6M RA was applied from 60% epiboly until 10 ss, then washed out. Embryos were then fixed at 24 hpf before whole-mount in situ hybridization with fli1 riboprobe. Representative embryos from 1 of 3 experiments are shown.
