Nonerythroid cells accumulate in MASL1-knockdown CD34⁺ cells following EPO-induced erythroid differentiation. A hematopathologist, blinded to experimental details, reviewed all coded slides and quantitated myeloid and erythroid cells at day 3, 5, 7, and 14 of EPO-induced erythroid differentiation in mock-, control shRNA-, MASL1 siRNA-, or MASL1 shRNA-transfected CD34⁺ cells. More than 200 cells were scored for each sample in triplicate individual experiments. The data represent the mean values of 3 independent experiments.