Deregulation of Wnt pathway gene expression in CLL. (A) Detection of LEF1, WNT3, TCF4 [ITF-2], and CCND2 mRNA levels by quantitative PCR in leukemic or normal B cells from 8 CLL patients and 3 healthy donor samples, respectively. Transcript levels were normalized to 18s rRNA, and relative fold change was calculated using the 2−ΔΔCT method. *P < .05. Error bars represent SEM. (B) Detection of IGFBP-4 protein secretion into culture media by ELISA in B cells from 6 CLL patients and 3 healthy donor samples. The dashed line at 5 ng/mL indicates the ELISA threshold level of IGFBP-4 detection, and the horizontal lines indicate the mean of each group.