Figure 2
Effects of DC activation on ISCOMATRIX facilitated HLA-A2-restricted cross-presentation of NY-ESO-1 or Melan-A. Immature MoDCs (1 × 105) were either unpulsed or pulsed with 0.5 to 10 μg/mL of (A) NY-ESO-1 or (B) Melan-A protein together with 10 μg/mL ISCOMATRIX adjuvant in the absence or presence of 100 ng/mL IFN-γ, 2 μg/mL CD40L, 100 ng/mL LPS, or 1800 U/mL IFN-α in 96-well, round-bottom plates for 18 hours. After being washed, MoDCs were cocultured with HLA-A2-restricted NY-ESO-1 or Melan-A-specific CTL lines for 4 hours in the presence of 10 μg/mL BFA. A standard ICS was then performed, and IFN-γ levels were assessed by flow cytometry. Data are mean ± SD of 3 separate donors.
