Viral genome content of control and FX-binding ablated Ad vectors 48 hours after administration. Viral genome content of liver (A) and spleen (B) from control (CL−) or macrophage-depleted (CL+) mice 48 hours after injection with increasing doses of Ad5, Ad5-HVR7(Ad26), or Ad5-HVR5*7*E451Q. Ad genomes were detected in liver and spleen after DNA extraction and analysis performed by quantitative PCR. (*P < .05 vs Ad5, n = 5).