Transduction profiles of Ad5, Ad5-HVR7(Ad26), and Ad5-HVR5*7*E451Q in control (CL−) or macrophage-depleted (CL+) mice. β-galactosidase expression was quantified by ELISA and normalized to total protein content. Animals were killed 48 hours after injection and liver and spleen were harvested for analysis. Liver (A), spleen (B), lung (C), and heart (D) transduction profile at increasing doses (1 × 10¹⁰, 3 × 10¹⁰, and 1 × 10¹¹ vp/mouse; *P < .05 vs Ad5, n = 5).