Colocalization of viral transgene expression with spleen cell markers after treatment with clodronate liposomes. Frozen spleen sections (6 μm) were stained with antibodies for SIGNR1, CD11b, CD11c, B220, ER-TR7, and MAdCAM-1 in combination with an antibody to viral transgene, β-galactosidase (Table 1). Single stained and merged images are presented. An enlarged portion of each merged image is displayed in the far right panel, highlighted by a white box. White arrows indicate regions of colocalization (in yellow). WP indicates White pulp; MZ, Marginal zone, and RP, Red pulp. Images are representative of multiple fields of view from 2-4 different animals.